9W29

Structure of Au bound to human heavy chain ferritin nanocage.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2023-07-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	F 4 3 2
Unit cell lengths	183.770, 183.770, 183.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.160 - 1.990
R-factor	0.2196
Rwork	0.218
R-free	0.24650
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.808
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.160	2.062
High resolution limit [Å]	1.990	1.990
Rmerge	0.408	4.424
Rmeas	0.411
Rpim	0.047
Number of reflections	17840	1588
<I/σ(I)>	13.23
Completeness [%]	93.7
Redundancy	74.6
CC(1/2)	0.998	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	2 M MgCl2, 0.1 M Bicine, pH 9.0