9W1U

Crystal structure of mutant apo LC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2025-05-08
Detector	RIGAKU
Wavelength(s)	1.54178
Spacegroup name	P 65
Unit cell lengths	74.056, 74.056, 42.303
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.240 - 1.600
R-factor	0.1929
Rwork	0.192
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zjd
RMSD bond length	0.007
RMSD bond angle	0.963
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.047
Number of reflections	17653	1750
<I/σ(I)>	53.69
Completeness [%]	99.0
Redundancy	18.5
CC(1/2)	1.000	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	200 mM Ammonium Sulphate 25% PEG 4000 100 mM Sodium acetate pH 4.6