9W1S

Crystal structure of AtNUT-AtBAK1-AtSCREW1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-09-07
Detector	PSI PILATUS 6M
Wavelength(s)	0.978797
Spacegroup name	P 2 21 21
Unit cell lengths	64.414, 136.781, 298.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.250 - 3.400
R-factor	0.231
Rwork	0.229
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.494
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.490
High resolution limit [Å]	3.400	3.400
Number of reflections	37095	3627
<I/σ(I)>	9.3
Completeness [%]	99.5
Redundancy	4.2
CC(1/2)	0.960	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		290.15	0.2 M potassium chloride, 20% (w/v) PEG3350