9VZC
Doc and Phd complex from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2018-02-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.035, 77.278, 58.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.450 - 1.800 |
| R-factor | 0.18324 |
| Rwork | 0.181 |
| R-free | 0.21716 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.734 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.310 | 1.830 |
| High resolution limit [Å] | 1.796 | 1.796 |
| Rmerge | 0.169 | 0.665 |
| Number of reflections | 31528 | 767 |
| <I/σ(I)> | 15.63 | 2.15 |
| Completeness [%] | 93.2 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.967 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.05M Citric acid, 0.05M BIS-TRIS propane pH 5.0, 16% w/v Polyethylene glycol 3350 |
