9VYB
Antitoxin Phd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2024-09-05 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.370, 47.760, 48.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.980 - 2.120 |
| R-factor | 0.23561 |
| Rwork | 0.234 |
| R-free | 0.26946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.637 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.980 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 6172 | 494 |
| <I/σ(I)> | 25.8 | 8.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 12.8 | |
| CC(1/2) | 0.990 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.12M Ethylene glycols, 0.1M Buffer System 3, 30 % v/v Precipitant Mix 2 |
