9VY7

Structure of MIF binding with Lanthanum ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979176
Spacegroup name	C 1 2 1
Unit cell lengths	70.896, 40.741, 56.416
Unit cell angles	90.00, 112.52, 90.00

Refinement procedure

Resolution	26.060 - 1.250
R-factor	0.1211
Rwork	0.121
R-free	0.12890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.384
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.110	52.110	1.260
High resolution limit [Å]	1.190	3.520	1.190
Rmerge	0.073
Rmeas	0.081
Rpim	0.035	0.029	0.310
Number of reflections	39396	1574	2135
<I/σ(I)>	16.6
Completeness [%]	82.4
Redundancy	4.6
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350