9VY5

Structure of MIF binding with Terbium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979176
Spacegroup name	P 21 21 2
Unit cell lengths	71.598, 120.081, 36.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.940 - 1.580
R-factor	0.1804
Rwork	0.179
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.887
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.280	36.280	1.570
High resolution limit [Å]	1.550	6.530	1.550
Rmerge	0.190	0.155	0.866
Rmeas	0.207
Rpim	0.083
Number of reflections	46026	618	3027
<I/σ(I)>	7.3
Completeness [%]	99.0
Redundancy	11.2
CC(1/2)	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% v/v 2-propanol, 0.1 M BICINE pH 8.5, 30% polyethylene glycol 3,350