9VY4

Structure of MIF binding with Ytterbium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-30
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979176
Spacegroup name	P 21 21 2
Unit cell lengths	72.222, 120.294, 36.011
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.060 - 1.210
R-factor	0.1249
Rwork	0.124
R-free	0.13790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.535
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.920	61.920	1.280
High resolution limit [Å]	1.210	3.610	1.210
Rmerge	0.097	0.047	0.552
Rmeas	0.102
Rpim	0.030
Number of reflections	73230	3317	2751
<I/σ(I)>	21.9
Completeness [%]	76.8
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES pH 6.5, 25% w/v Polyethylene glycol 4,000, 0.7% v/v 1-butanol