9VY3

Structure of apo MIF (Lanpepsy)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-28
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979176
Spacegroup name	P 21 21 21
Unit cell lengths	70.704, 70.843, 130.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.050 - 2.080
R-factor	0.2204
Rwork	0.219
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.729
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.720	39.720	2.170
High resolution limit [Å]	2.080	7.880	2.100
Rmerge	0.168
Rmeas	0.176
Rpim	0.050	0.033	0.271
Number of reflections	40085	701	3554
<I/σ(I)>	9.88
Completeness [%]	99.7
Redundancy	12.5
CC(1/2)	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Ammonium fluoride, 20% w/v Polyethylene glycol 3,350