9VXL

CD38 in complex with 028 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2021-10-13
Detector	BRUKER PHOTON 100
Wavelength(s)	0.9785
Spacegroup name	P 21 21 2
Unit cell lengths	54.868, 235.879, 54.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.170 - 3.150
R-factor	0.2361
Rwork	0.233
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.170	3.260
High resolution limit [Å]	3.150	3.150
Number of reflections	11963	1197
<I/σ(I)>	2
Completeness [%]	93.0
Redundancy	19.7
CC(1/2)	0.920	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.2 M lithium nitrate, and 20% PEG3350