9VWQ
Crystal structure of Nanobody Nb3.30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.298, 73.424, 43.901 |
| Unit cell angles | 90.00, 109.68, 90.00 |
Refinement procedure
| Resolution | 32.060 - 1.300 |
| R-factor | 0.1792 |
| Rwork | 0.179 |
| R-free | 0.19150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.892 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.060 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 141410 | 563 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 97.5 | |
| Redundancy | 3 | |
| CC(1/2) | 0.999 | 0.512 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES sodium pH 7.5, 1.4 M sodium citrate tribasic dihydrate |
