9VWB
HamA, a Heme-Oxygenase-Like Enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-23 |
| Detector | RIGAKU HyPix-Arc 150 |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 101.551, 101.551, 46.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.520 - 2.000 |
| R-factor | 0.17279 |
| Rwork | 0.170 |
| R-free | 0.21936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.425 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.520 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.244 | 1.388 |
| Rmeas | 0.255 | 1.451 |
| Rpim | 0.072 | 0.417 |
| Total number of observations | 13001 | |
| Number of reflections | 16978 | 1195 |
| <I/σ(I)> | 12.1 | 5.5 |
| Completeness [%] | 99.2 | |
| Redundancy | 11.7 | 10.9 |
| CC(1/2) | 0.996 | 0.922 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.1 M sodium acetate trihydrate pH 5.0 2.85 M sodium formate 5% glycerol |
