9VW7

NAD(P)-dependent oxidoreductase from Kutzneria albida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	117.395, 117.395, 99.569
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.540 - 2.499
Rwork	0.160
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.552
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	MrBUMP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.840	2.590
High resolution limit [Å]	2.499	2.499
Rmerge	0.057
Number of reflections	24403	2234
<I/σ(I)>	13.3
Completeness [%]	98.9
Redundancy	1.9
CC(1/2)	0.995	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		279.15	1.0 M Ammonium Sulfate, 0.1 M HEPES:NaOH pH 7.0, and 0.5% (w/v) PEG 8000