9VVT
Crystal structure of the LysR-type transcriptional regulator CutR from mycobacterium sp. strain JC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-14 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.500, 92.079, 126.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.260 - 2.500 |
| R-factor | 0.21479 |
| Rwork | 0.212 |
| R-free | 0.26804 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.115 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.113 | 0.075 | 0.487 |
| Total number of observations | 375109 | ||
| Number of reflections | 26309 | 2815 | 2581 |
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 14.3 | 13 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294.15 | 5% PEG 8000, 0.1 M sodium acetate, 4% glycol |
