9VV4
Research on the Structure and Function of Thioredoxin Reductase NTRC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2021-07-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.374, 155.113, 130.491 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.550 |
| Rwork | 0.227 |
| R-free | 0.26880 |
| Structure solution method | AB INITIO PHASING |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 10.000 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Number of reflections | 13341 | 12968 |
| <I/σ(I)> | 7.22 | |
| Completeness [%] | 97.3 | |
| Redundancy | 5.8 | |
| CC(1/2) | 0.975 | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 25% PEG 8000 , 0.2M NaCl , 0.1M Tris 8.5 |
