9VUP

SFTSV-Gn/N2D2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2024-05-30
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	58.269, 126.908, 76.426
Unit cell angles	90.00, 97.00, 90.00

Refinement procedure

Resolution	29.730 - 2.520
R-factor	0.1993
Rwork	0.197
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.323
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.109	0.056	0.523
Rmeas	0.125	0.064	0.605
Rpim	0.060	0.031	0.295
Total number of observations	114839
Number of reflections	31959	3068	3254
<I/σ(I)>	12
Completeness [%]	87.6	82.5	89.4
Redundancy	3.6	3.5	3.3
CC(1/2)	0.985	0.996	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		300	50 mM sodium formate (pH 6.8), 15% (w/v) PEG 3350