9VUD
SFTSV-Gn/N4E8 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2024-06-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 95.336, 95.336, 154.063 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 - 2.690 |
| R-factor | 0.2089 |
| Rwork | 0.207 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.690 | 5.810 | 2.700 |
| Rmerge | 0.079 | 0.046 | 0.557 |
| Rmeas | 0.084 | 0.049 | 0.593 |
| Rpim | 0.029 | 0.018 | 0.196 |
| Number of reflections | 19438 | 2004 | 1931 |
| <I/σ(I)> | 16.4 | ||
| Completeness [%] | 95.2 | 89.5 | 98 |
| Redundancy | 7.8 | 6.7 | 8 |
| CC(1/2) | 0.997 | 0.998 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289.15 | 0.1 M trisodium citrate dihydrate/20% (v/v) 2-propanol/10% (w/v) PEG 4000 |
