9VTV
Chitinase TfeC from Yersinia pseudotuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.913, 63.926, 77.947 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 49.429 - 1.900 |
| R-factor | 0.1926 |
| Rwork | 0.184 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.753 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.7.0) |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.429 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 35572 | 10575 |
| <I/σ(I)> | 9.8 | 4.2 |
| Completeness [%] | 98.9 | 88.4 |
| Redundancy | 6.6 | 5.3 |
| CC(1/2) | 0.991 | 0.908 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 0.2M Potassium Na tartrate tetrahydrate, 20% PEG 3350 |
