9VSF

GH64 Beta-1,3-glucanase from Candidatus Saccharibacteria bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-21
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979145
Spacegroup name	I 1 2 1
Unit cell lengths	112.630, 100.590, 113.168
Unit cell angles	90.00, 90.69, 90.00

Refinement procedure

Resolution	56.310 - 2.130
R-factor	0.20433
Rwork	0.201
R-free	0.25907
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.806
Data reduction software	XDS
Data scaling software	Aimless (1.12.16)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.310	2.180
High resolution limit [Å]	2.130	2.130
Rmerge	0.161	1.108
Rmeas	0.174	1.196
Rpim	0.067	0.447
Number of reflections	70371	4509
<I/σ(I)>	9.8	2.1
Completeness [%]	99.8	100
Redundancy	6.8	7
CC(1/2)	0.996	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	298	0.1M HEPES, 0.8M Potassium sodium tartrate