9VS2

Crystal structure of FOXC2/NFAT1 complex bound to ARRE3-P DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	67.714, 79.510, 120.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.401 - 2.650
R-factor	0.2309
Rwork	0.228
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.755
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	2.750
High resolution limit [Å]	2.650	2.650
Rmerge	0.300	1.190
Number of reflections	18641	1496
<I/σ(I)>	6.84	2.27
Completeness [%]	95.5	77.31
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	50 mM MES 5.93, 200 mM NaCl, 10 mM MgCl2, 1 mM TCEP and 12-15% PEG 4K (w/v)