9VR2
The crystal structure of MetRS-like protein from Streptomyces candidus in complex with L-Glu and L-N6-OH-lysine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-15 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.583, 50.534, 106.613 |
| Unit cell angles | 90.00, 89.82, 90.00 |
Refinement procedure
| Resolution | 41.670 - 2.450 |
| R-factor | 0.17461435973 |
| Rwork | 0.172 |
| R-free | 0.21786 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.290 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.671 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 40316 | 40316 |
| <I/σ(I)> | 6.68 | |
| Completeness [%] | 97.7 | |
| Redundancy | 6.68 | |
| CC(1/2) | 0.937 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 100mm Tris ph8.5, 200 mM MgCl2, 20% PEG 8000 |
