9VQN

Crystal structure of Se-Met SSA1633 from Streptococcus sanguinis (Phase determination model)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	37.288, 58.883, 214.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.760 - 2.200
R-factor	0.2005
Rwork	0.199
R-free	0.22560
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.580
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.460	1.870
High resolution limit [Å]	1.830	1.830
Number of reflections	24939	1715
<I/σ(I)>	7.2
Completeness [%]	95.0
Redundancy	6.2
CC(1/2)	0.995	0.176

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Potassium sodium tartrate, 0.1M HEPES-HCl