9VQJ
The crystal structure of MetRs-like protein from Streptomyces candidus bound to L-Glu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-15 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.518, 50.290, 114.127 |
| Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
| Resolution | 49.358 - 1.956 |
| R-factor | 0.167931492525 |
| Rwork | 0.167 |
| R-free | 0.21279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.360 | 2.010 |
| High resolution limit [Å] | 1.955 | 1.955 |
| Number of reflections | 87158 | 87158 |
| <I/σ(I)> | 2.08 | |
| Completeness [%] | 95.2 | |
| Redundancy | 6.72 | |
| CC(1/2) | 0.996 | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 100mm Tris ph8.5, 200 mM MgCl2, 20% PEG 8000 |
