9VPF

Crystal structure of D-aspartate oxidase from Cryptococcus humicola UJ1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	84.983, 109.338, 189.401
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.350 - 1.700
R-factor	0.19093
Rwork	0.190
R-free	0.21559
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.403
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.350	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.069	0.718
Rmeas	0.074	0.772
Rpim	0.027	0.281
Number of reflections	193803	27993
<I/σ(I)>	19.1
Completeness [%]	100.0
Redundancy	7.3
CC(1/2)	0.999	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	PEG 1540, PEG 400, Ammonium Fluoride, MPD