9VPA

GH64 family beta-1,3-glucanase from Massilia violaceinigra

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-21
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979145
Spacegroup name	P 62
Unit cell lengths	126.260, 126.260, 71.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.360 - 2.300
R-factor	0.16451
Rwork	0.164
R-free	0.17962
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.090
Data reduction software	iMOSFLM (7.4.0)
Data scaling software	SCALA (8.0.019)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.150	0.701
Rmeas	0.157	0.736
Rpim	0.048	0.223
Total number of observations	569708	86359
Number of reflections	55571	8198
<I/σ(I)>	17	7.1
Completeness [%]	98.0
Redundancy	10.3	10.5
CC(1/2)	0.996	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	298	2.8M NaAc