9VP9

GH64 family beta-1,3-glucanase from Massilia violaceinigra in complex with a glucose disaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-27
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979145
Spacegroup name	P 62
Unit cell lengths	126.670, 126.670, 71.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.460 - 1.990
R-factor	0.17383
Rwork	0.172
R-free	0.20499
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.705
Data reduction software	autoPROC (1.0.5)
Data scaling software	xia2 (3.6.1)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.460	2.040
High resolution limit [Å]	1.990	1.990
Rmerge	0.200	1.788
Rmeas	0.359	1.000
Rpim	0.081	0.401
Number of reflections	44713	3303
<I/σ(I)>	20.1
Completeness [%]	100.0	100
Redundancy	20	15
CC(1/2)	0.996	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	298	2.8M NaAc