9VOQ

Engineered endo-1,3-beta-glucanase from Cellulosimicrobium cellulans crystallized at pH6.2,CcGluECDMH1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-15
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979145
Spacegroup name	P 63
Unit cell lengths	73.248, 73.248, 62.264
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.430 - 1.280
R-factor	0.15441
Rwork	0.153
R-free	0.17495
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.900
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.931	1.810
High resolution limit [Å]	1.280	1.760
Rmerge	0.137	0.137
Rmeas		0.074
Number of reflections	34530	638
<I/σ(I)>	10.4	10.4
Completeness [%]	99.9	99.7
Redundancy	4.1
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.2	298	0.2M (NH4)2SO4 0.1M C2H12AsNaO5 pH6.2 30%PEG8000