9VO7

Crystal structure of HuHF-C1, a HuHF varitant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9785
Spacegroup name	F 4 3 2
Unit cell lengths	179.104, 179.104, 179.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.090 - 2.600
R-factor	0.219
Rwork	0.215
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.484
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.114	0.048	0.632
Rmeas	0.117	0.050	0.649
Rpim	0.025	0.011	0.144
Total number of observations	176897
Number of reflections	8078	483	391
<I/σ(I)>	7
Completeness [%]	100.0	99.8	100
Redundancy	21.9	18.6	19.5
CC(1/2)	0.999	0.999	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bincine MgCl2