9VO5
X-ray structure of Clostridium perfringens pili CppB-D3D4D5-CppA covalent complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.360, 70.710, 110.590 |
| Unit cell angles | 93.63, 107.20, 89.90 |
Refinement procedure
| Resolution | 48.360 - 1.850 |
| R-factor | 0.19058 |
| Rwork | 0.189 |
| R-free | 0.21619 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.500 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.360 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 156433 | 20342 |
| <I/σ(I)> | 8.98 | |
| Completeness [%] | 95.6 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.996 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M Magnesium formate dihydrate, 20% w/v Polyethylene glycol 3,350 |
