9VNO

The crystal structure of the Ehd3 tandem PHD-H3K4me1 complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	I 41
Unit cell lengths	74.640, 74.640, 73.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.390 - 1.500
R-factor	0.1624
Rwork	0.162
R-free	0.17760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.657
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.610
High resolution limit [Å]	1.500	1.500
Rmerge	0.091	0.432
Rmeas	0.096	0.493
Rpim	0.030	0.229
Number of reflections	30102	2836
<I/σ(I)>	6.4
Completeness [%]	99.6	96.6
Redundancy	8.6	4
CC(1/2)	0.999	0.317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M Sodium phosphate dibasic dehydrate, 20% PEG3350