9VN8

Glucooligosaccharide oxidase from Hapsidospora chrysogenum, recombinant wild-type expressed in Komagataella phaffii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2024-06-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	72.055, 93.547, 100.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.030 - 1.650
R-factor	0.1665
Rwork	0.165
R-free	0.19020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.006
RMSD bond angle	0.904
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (8.0.019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.630	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.092	0.717
Rpim	0.039	0.299
Number of reflections	82243	4031
<I/σ(I)>	14	2.6
Completeness [%]	99.9	100
Redundancy	6.5	6.6
CC(1/2)	0.998	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	Tris-HCl, Ammonium sulfate, Bis-Tris