9VMQ

Crystal structure of Dot/Icm T4SS effector Ceg10(C159S)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	50.888, 54.596, 82.942
Unit cell angles	91.40, 91.11, 91.93

Refinement procedure

Resolution	20.000 - 1.790
R-factor	0.15612
Rwork	0.154
R-free	0.19890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.307
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.890	1.880
High resolution limit [Å]	1.790	1.790
Number of reflections	81024	11747
<I/σ(I)>	5.5
Completeness [%]	95.9
Redundancy	2.9
CC(1/2)	0.995	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.1M sodium formate, 10% PEG3350, 15mM EDTA