9VME

Structure of a triple-helix region of human collagen type VIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-26
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.54187
Spacegroup name	C 1 2 1
Unit cell lengths	54.510, 16.960, 59.310
Unit cell angles	90.00, 101.79, 90.00

Refinement procedure

Resolution	22.560 - 1.940
R-factor	0.1911
Rwork	0.189
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.846
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.560	2.000
High resolution limit [Å]	1.940	1.940
Number of reflections	3896	258
<I/σ(I)>	36.34
Completeness [%]	93.4
Redundancy	7.2
CC(1/2)	0.999	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.05 M Ammonium Sulfate, 0.05 M Bis-Tris: HCl, pH 6.5, 30 % (v/v) Pentaerythritol Ethoxylate (15/4_EO/OH)