9VMB

The X-RAY co-crystal structure of human FGFR3 and Compound 10t

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	80
Detector technology	CCD
Collection date	2025-05-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	58.345, 112.843, 87.515
Unit cell angles	90.00, 94.93, 90.00

Refinement procedure

Resolution	19.100 - 1.970
R-factor	0.1897
Rwork	0.187
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.065
Data reduction software	DIALS
Data scaling software	EVAL15
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.900	2.040
High resolution limit [Å]	1.970	1.970
Rmerge	0.247	0.929
Number of reflections	79399	7793
<I/σ(I)>	5.29	1.84
Completeness [%]	99.5
Redundancy	5.8
CC(1/2)	0.892	0.202

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% (w/v) PEG 3350, 0.2 M MgCl2, and 0.1 M HEPES, pH 7.5