9VLR

Acinetobacter baumannii membrane-bound lytic transglycosylase B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	75.490, 75.490, 175.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.329 - 1.790
Rwork	0.182
R-free	0.20460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.487
Data scaling software	XDS
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.330	1.854
High resolution limit [Å]	1.790	1.790
Number of reflections	48656	4762
<I/σ(I)>	20.21
Completeness [%]	99.9
Redundancy	26.6
CC(1/2)	0.999	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	25% (w/v) PEG-1500, 0.1M MMT Buffer pH6.5