9VLI
Crystal structure of Bacillus subtilis DegQ S25L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2017-05-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.03912 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.715, 41.920, 132.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.144 - 1.600 |
| Rwork | 0.210 |
| R-free | 0.25450 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.085 | 0.543 |
| Number of reflections | 21288 | 2086 |
| <I/σ(I)> | 19.8 | 6.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 15%(v/v) polyethylene glycol 3350, 5 mM magnesium chloride, 0.1 M HEPES |
