9VLH
Crystal structure of Bacillus subtilis DegQ tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-12-09 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.548, 52.271, 53.098 |
| Unit cell angles | 87.44, 89.57, 89.94 |
Refinement procedure
| Resolution | 38.100 - 2.300 |
| Rwork | 0.220 |
| R-free | 0.25420 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.349 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.049 | 0.197 |
| Rmeas | 0.059 | 0.235 |
| Rpim | 0.032 | 0.126 |
| Number of reflections | 15256 | 764 |
| <I/σ(I)> | 12.2 | 4.2 |
| Completeness [%] | 95.4 | 81.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 15%(v/v) polyethylene glycol 3350, 5 mM magnesium chloride, 0.1 M HEPES |
