9VLE
hAGO2-MID in complex with a chemical modified uridine monophosphate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.549, 46.635, 66.059 |
| Unit cell angles | 86.94, 74.04, 84.57 |
Refinement procedure
| Resolution | 38.090 - 1.990 |
| R-factor | 0.20601 |
| Rwork | 0.203 |
| R-free | 0.25915 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.621 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.410 | 1.790 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 30657 | 30657 |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 84.4 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.984 | 0.424 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.25M Ammonium sulfate 0.1M Sodium cacodylate trihydrate pH 6.5 30% w/v Polyethylene glycol 8,000 |
