9VLD
hAGO2-MID in complex with a chemical modified uridine monophosphate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.693, 47.311, 66.431 |
| Unit cell angles | 86.34, 73.58, 84.07 |
Refinement procedure
| Resolution | 47.070 - 2.050 |
| R-factor | 0.20737 |
| Rwork | 0.205 |
| R-free | 0.25439 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.987 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.070 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 28673 | 28673 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 96.3 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.998 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.25M Ammonium sulfate 0.1M Sodium cacodylate trihydrate pH 6.5 30% w/v Polyethylene glycol 8,000 |
