9VLB

Crystal Structure of a GDP-bound Bombyx mori RabX6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-11
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	77.593, 109.735, 40.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.800 - 3.100
R-factor	0.2387
Rwork	0.236
R-free	0.29120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z0a
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.800	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	6694	669
<I/σ(I)>	12.75
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.999	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.4	0.2 M MgCl2, 0.1 M Tris-HCl pH8.5 ,25% PEG 3350, 4% Polypropylene glycol P400