9VLA
Crystal structure of PNGase Bb in complex with MUXF glycopeptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.720, 97.890, 96.320 |
| Unit cell angles | 90.00, 122.02, 90.00 |
Refinement procedure
| Resolution | 33.470 - 2.160 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.750 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.106 | 1.158 |
| Number of reflections | 57354 | 4255 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.998 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 1M Sodium citrate tribasic,0.1M Tris base/Hydrochloric acid, 200mM Sodium Chloride |
