9VJC

Crystal structure of Sofic H194A mutant from Escherichia coli H397

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97861
Spacegroup name	P 1 21 1
Unit cell lengths	68.595, 58.234, 102.863
Unit cell angles	90.00, 93.57, 90.00

Refinement procedure

Resolution	55.390 - 2.280
R-factor	0.2148
Rwork	0.212
R-free	0.26660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.964
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.390	2.364
High resolution limit [Å]	2.280	2.283
Number of reflections	35092	3222
<I/σ(I)>	11.5
Completeness [%]	94.4
Redundancy	6.3
CC(1/2)	0.995	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	0.1M HEPES pH7.5, 20% PEG1500, 0.2M L-Proline