9VI7

Acinetobacter baumannii membrane-bound lytic murein transglycosylase C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	58.740, 105.880, 130.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.470 - 2.490
R-factor	0.22
Rwork	0.217
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.980
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.488	2.579
High resolution limit [Å]	2.490	2.490
Number of reflections	29242	2893
<I/σ(I)>	14.42
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	0.999	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	60% (v/v) Polypropylene glycol 400, 0.1M Tris pH8.0