9VHZ

Structure of MPXV M1R and hmMM1-40 Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	61.361, 72.353, 170.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.180 - 2.800
R-factor	0.2118
Rwork	0.207
R-free	0.29570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.155
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.290	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	18631	949
<I/σ(I)>	8.2
Completeness [%]	96.0
Redundancy	7.3
CC(1/2)	0.986	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	1% w/v Tryptone, 0.05 M HEPES sodium pH 7.0, 20% w/v Polyethylene glycol 3,350