9VHM
structure of CC2D1A (coiled-coil and C2 domain containing - 1A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-08 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.385, 70.257, 201.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.430 - 2.300 |
| R-factor | 0.2185 |
| Rwork | 0.215 |
| R-free | 0.27020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.570 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 38448 | 3353 |
| <I/σ(I)> | 29.2 | |
| Completeness [%] | 97.1 | 85.6 |
| Redundancy | 6 | 4.9 |
| CC(1/2) | 0.960 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Tris-HCl pH 8.5 and potassium thiocyanate |
