9VHC
ATP-dependent Clp endopeptidase proteolytic subunit ClpP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 252.981, 273.576, 249.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.730 - 3.030 |
| R-factor | 0.2167 |
| Rwork | 0.215 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.730 | 3.110 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Number of reflections | 166729 | 12292 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.5 | |
| CC(1/2) | 0.997 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 0.2 M ammonium acetate, 0.1 M tris pH 8.0, PEG 10000 |
