9VGJ
An Artificially Designed Monomeric All-beta-Sheet Protein with 86.13% beta-Content
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 65 |
| Unit cell lengths | 61.396, 61.396, 48.595 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.590 - 1.300 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.784 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.170 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.073 | 0.806 |
| Rpim | 0.026 | 0.592 |
| Number of reflections | 25493 | 1216 |
| <I/σ(I)> | 20.9 | 2 |
| Completeness [%] | 99.0 | |
| Redundancy | 15.4 | |
| CC(1/2) | 0.999 | 0.625 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Sodium citrate pH4.5 20% w/v PEG4000 |
