9VG8

Crystal structure of Phaeodactylibacter sp. phosphoglucomutase in complex with magnesium ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97861
Spacegroup name	P 21 21 2
Unit cell lengths	61.850, 168.980, 59.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.490 - 1.650
R-factor	0.2227
Rwork	0.221
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.809
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.490	1.740
High resolution limit [Å]	1.650	1.650
Number of reflections	64621	7269
<I/σ(I)>	19.2
Completeness [%]	86.3
Redundancy	10.4
CC(1/2)	0.999	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 6.0, 24% v/v Polyethylene glycol 400 1 mM Mg2+