9VG4

Structural complex of FTO bound with 8j

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97923
Spacegroup name	H 3
Unit cell lengths	141.830, 141.830, 83.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.920 - 2.300
R-factor	0.20486
Rwork	0.203
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.575
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.920	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.044	0.044
Number of reflections	26483	26483
<I/σ(I)>	11.2
Completeness [%]	99.8
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	100 mM sodium citrate, pH 5.6, 11.5% (w/v) polyethylene glycol (PEG) 3350,8% isopropanol.