9VG0

SIRT2 structure in complex with H3K18myr peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	36.152, 73.504, 55.731
Unit cell angles	90.00, 94.09, 90.00

Refinement procedure

Resolution	27.810 - 1.610
Rwork	0.188
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x3o
RMSD bond length	0.013
RMSD bond angle	1.903
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.590	1.640
High resolution limit [Å]	1.610	1.610
Number of reflections	34600	1925
<I/σ(I)>	17.1
Completeness [%]	91.8
Redundancy	6.4
CC(1/2)	0.997	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Tris 8.0, 25% PEG 2000MME